Construction of tests

Since working with Salmonella was not possible, an Enterococcus faecium (ATCC® 8459™) was used for the experiment. This type corresponds largely in its behavior to Salmonella ssp. In order to produce a typical contaminated goods with germs, sesame seeds were loaded under standard conditions in the pilot plant with 104 CFU /g² [15,000 g Sesame with 150 ml of a solution containing approximately 106 cfu/g E. faecium]. This goods were three times subjected immediately to the infrared treatment at 105°C. In each case four samples were taken. Afterwards the samples were given to microbiological examination, which were analyzed with three different methods.
(Parallel two samples from an IR treated sesame seed without germ injection and two samples of germ added goods without IR-treatment were produced and analyzed.)
The sample preparation (determining the bacteria) of all samples was carried out within 24 hours after inoculation and Treatment.

Results and evaluation

The Reducing of the “Compare germ” Enterococcus faecium (ATCC® 8459™) is at each sample – performed by the processes, which is described above, safely at absent in 25g. However, this also corresponds to the effects which are typical for a natural contamination with salmonella.

Summary

The germ reduction with infrared light at FS-IRD is able even with seed types which correspond to those of Salmonella, this certainly kill so that they are not detectable in 25g.

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